Peripheral arterial occlusive disease model(mice,rats)

Effective treatment for serious limb ischemia resulted from diabetes mellitus is few, so speedy developing new drugs are expected.
Limb ischemia models with one side femoral arterial and venous removed are often used to evaluate pharmacological effects of such these drugs.
Following items are evaluated; skin blood volume, macroscopically observed lesions, histopathological analysis such as measurement number of capillary, gait analysis.